Determination of the antiallergic agent KB-2413 in plasma by means of capillary gas chromatography with a nitrogen-sensitive detector.
- 31 December 1985
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (3) , 1168-1171
- https://doi.org/10.1248/cpb.34.1168
Abstract
A gas chromatographic method for the quantitative determination of the new antiallergic agent, 1-(2-ethoxyethyl)-2-(4-methyl-1-homopiperazinyl)benzimidazole difumarate (KB-2413), in plasma has been developed. This method, which was based on solvent extraction and flexible fused-silica capillary gas chromatography with a nitrogen-sensitive detector and a moving needle solvent cut sample injector, is simple, sensitive and selective. The detection limit was about 1 ng (as free base)/ml and the calibration curve was linear over the range of 2 to 100 ng/ml. Moreover, the intra- and inter-assay coefficients of variation for the determination of KB-2413 wer 3.1-4.0% and 1.5-6.7%, respectively. This assay was applied to the determination of the plasma concentration of KB-2413 base after oral administration to dogs.This publication has 1 reference indexed in Scilit:
- Application of fused-silica capillary gas chromatography to the analysis of underivatized drugsJournal of Chromatography A, 1982