Mutant glycosyltransferases assist in the development of a targeted drug delivery system and contrast agents for MRI
Open Access
- 1 March 2006
- journal article
- review article
- Published by Springer Nature in The AAPS Journal
- Vol. 8 (1) , E190-E195
- https://doi.org/10.1208/aapsj080123
Abstract
The availability of structural information on glycosyltransferases is beginning to make structure-based reengineering of these enzymes possible. Mutant glycosyltransferases have been generated that can transfer a sugar residue with a chemically reactive unique functional group to a sugar moiety of glycoproteins, glycolipids, and proteoglycans (glycoconjugates). The presence of modified sugar moiety on a glycoprotein makes it possible to link bioactive molecules via modified glycan chains, thereby assisting in the assembly of bionanoparticles that are useful for developing the targeted drug delivery system and contrast agents for magnetic resonance imaging. The reengineered recombinant glycosyltransferases also make it possible to (1) remodel the oligosaccharide chains of glycoprotein drugs, and (2) synthesize oligosaccharides for vaccine development.Keywords
This publication has 64 references indexed in Scilit:
- Mutation of Arginine 228 to Lysine Enhances the Glucosyltransferase Activity of Bovine β-1,4-Galactosyltransferase I,,Biochemistry, 2005
- One-Step Synthesis of Labeled Sugar Nucleotides for Protein O-GlcNAc Modification Studies by Chemical Function Analysis of an Archaeal ProteinJournal of the American Chemical Society, 2004
- Structure and catalytic cycle of β-1,4-galactosyltransferaseCurrent Opinion in Structural Biology, 2004
- History of lectins: from hemagglutinins to biological recognition moleculesGlycobiology, 2004
- Galactosyltransferase—still up and runningBiochimie, 2003
- Identification of a Drosophila Gene Encoding Xylosylprotein β4-Galactosyltransferase That Is Essential for the Synthesis of Glycosaminoglycans and for MorphogenesisJournal of Biological Chemistry, 2002
- Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functionsBiochimica et Biophysica Acta (BBA) - General Subjects, 1999
- Roles for glycosylation of cell surface receptors involved in cellular immune recognitionJournal of Molecular Biology, 1999
- Cell-Type Specific and Ligand Specific Enhancement of Cellular Uptake of Oligodeoxynucleoside Methylphosphonates Covalently Linked with a Neoglycopeptide, YEE(ah-GalNAc)3Bioconjugate Chemistry, 1995
- Hepatic uptake of proteins coupled to fetuin glycopeptideBiochemical and Biophysical Research Communications, 1971