Micromethods for Analysis of Lipids in Endomyocardial Biopsy Specimens

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Abstract
Triglycerides and phospholipids extracted from endomyocardial biopsy specimens [human] were quantitated with sensitive photometric methods. The fatty acid composition of phospholipids was determined after separation of the methyl esters by gas chromatography. The degree of triglyceride accumulation was expressed as the molar ratio triglyceride/phospholipid since in most cases the phospholipid concentration is relatively constant. The representativity of the analysis of endomyocardial biopsy was studied by comparing lipid analyses of large and small myocardial specimens obtained at autopsy. The methods described may be valuable in studying metabolic disturbances in different cardiomyophaties.