Repair of double-strand breaks and lethal damage in DNA ofUstilago maydis
- 1 June 1980
- journal article
- research article
- Published by Hindawi Limited in Genetics Research
- Vol. 35 (3) , 291-307
- https://doi.org/10.1017/s0016672300014154
Abstract
SUMMARY: The size of nuclear DNA from wild-typeUstilago maydiswas determined to be approximately 6·09 ± 0·3 × 108daltons from neutral sucrose gradient sedimentation analysis. Following exposure to ionizing radiation the nuclear DNA size was reduced due to the production of double-strand breaks in the DNA. These breaks were repaired when the irradiated cells were incubated in medium for at least one hour after irradiation. The repair was seen as a shift in the DNA profile from a low molecular weight region where the control DNA sedimented. Inhibition of protein synthesis by cycloheximide prevented this type of repair. Blocking protein synthesis also decreased the survival of irradiated wild-type cells but not radiation-sensitive mutants. Protein synthesis was necessary within the first one and a half hours after irradiation for the survival of wild-type cells to be unaffected. The results provide additional evidence for an inducible repair process inU. maydis.This publication has 34 references indexed in Scilit:
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