BrdU‐Hoechst flow cytometry reveals regulation of human lymphocyte growth by donor‐age–related growth fraction and transition rate

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Abstract
An improved BrdU-Hoechst flow assay was applied to cell kinetic studies of human lymphocyte cultures during a 24–96 hr interval after PHA stimulation. The assay shows that the duration of the initial lag phase and the proportions of noncycling cells increase as a function of donor age, whereas the rates of transition from each cell cycle compartment to the next decrease. Cell cycle arrest occurs in the first S and G2 phase after stimulation of lymphocytes from a 75-year-old donor but not from younger donors. The data are consistent with several models of cell cycle kinetics, so long as these models are modified to include a fraction of noncycling cells in each cell cycle compartment.