ADP tethered to tyrosine‐β345 at the catalytic site of the bovine heart F1‐ATPase is converted to tethered AMP by Mg2+‐dependent hydrolysis when the enzyme is photoinactivated with 2‐N3‐ADP
- 20 June 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 347 (1) , 13-16
- https://doi.org/10.1016/0014-5793(94)00497-8
Abstract
Comparison of profiles of radioactive peptides resolved by HPLC from tryptic digests of the bovine heart F1‐ATPase depleted of nucleotides (nd‐MF1) which had been photoinactivated with 2‐N3‐[β‐32P]ADP, on the one hand, and 2‐[8‐3H]ADP, on the other, shows that the β phosphate of ADP tethered to tyrosine‐β345 is slowly hydrolyzed in the presence of Mg2+. When nd‐MF1 was photoinactivated with 2‐N3‐[8‐3H]ADP in the absence of Mg2+, hydrolysis of the β phosphate from ADP tethered to tyrosine‐β345 was not observed. Subsequent addition of Mg2+ initiated conversion of ADP tethered to tyrosine‐β345 to tethered AMP suggesting that functional groups at the catalytic site participate in the hydrolytic reaction.Keywords
This publication has 26 references indexed in Scilit:
- ATP synthesis in mitochondriaEuropean Journal of Biochemistry, 1993
- Functional nucleotide-binding domain in the F0F1-ATP synthase .alpha. subunit from the yeast Schizosaccharomyces pombeBiochemistry, 1993
- Differential nucleotide binding to catalytic and noncatalytic sites and related conformational changes involving .alpha./.beta.-subunit interactions as monitored by sensitive intrinsic-fluorescence in Schizosaccharomyces pombe mitochondrial F1Biochemistry, 1992
- Chapter 13 The reaction mechanism of F0F1ATP synthasesPublished by Elsevier ,1992
- The ADP that binds tightly to nucleotide-depleted mitochondrial F1-ATPase and inhibits catalysis is bound at a catalytic siteBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Site-directed mutagenesis of stable adenosine triphosphate synthaseBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1988
- Photoaffinity labeling of mitochondrial adenosine triphosphatase by [.alpha.-32P]2-azido ADPBiochemistry, 1985
- Measurement of protein using bicinchoninic acidAnalytical Biochemistry, 1985
- Tautomerism of 2-azidoadenine nucleotides Effects on enzyme kinetics and photoaffinity labelingBiochimica et Biophysica Acta (BBA) - General Subjects, 1984
- "Hysteretic" behavior and nucleotide binding sites of pig heart mitochondrial F1 adenosine 5'-triphosphataseBiochemistry, 1980