GRAFT-VERSUS-HOST REACTIVITY AND RENAL ALLOGRAFT SURVIVAL IN RATS GIVEN ALLOGENEIC SPLEEN CELLS OR SPLEEN ALLOGRAFTS

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Abstract
Selective recruitment of antigen-sensitive cells (ASC) into the spleen as a method of inducing specific suppression was attempted by i.v. injection of DA or Lewis spleen cells 24 h before a (DA .times. Lewis)F1 renal allograft into a Lewis or DA recipient, with or without a splenectomy. This led to suppression of rejection in the DA recipient and delayed rejection in the Lewis recipient. Splenectomy produced a minimal augmentation effect. Assay of graft-vs.-host (GVH) reactions in (DA .times. Lewis)F1 rats by a popliteal node assay showed that injection of allogeneic DA or Lewis spleen cells 48 h before the assay significantly reduced the reaction produced by node lymphocytes, but not spleen lymphocytes, suggesting a loss of ASC from the lymph nodes. Lewis spleen allografts did not produce such a significant reduction in the GVH reactivity of DA node lymphocytes as i.v. Lewis cells, whereas DA spleen allografts led to an increased GVH reactivity of Lewis node lymphocytes. It is not possible to attribute the suppression produced by the i.v. injection of allogeneic cells to selective recruitment of antigen-sensitive cells to the spleen.

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