TIME‐RESOLVED FLUORESCENCE STUDIES OF SPINACH CHLOROPLASTS–EVIDENCE FOR THE HETEROGENEOUS BIPARTITE MODEL

Abstract
Abstract—Fluorescence lifetimes of spinach chloroplasts were measured with a modelocked dye laser and time‐correlated single photon counting. Information about energy transport and functional organization of the chloroplasts is revealed by such time‐resolved fluorescence studies. Quenching experiments using treatment with UV light or the chemical agent dibromothymoquinone are consistent with the notion that there is heterogeneity associated with PS II units and that such heterogeneity is reflected over the entire time range of fluorescence decay, not just in a single component. Phosphorylation experiments were also carried out which permit us to relate these kinetic studies to previous steady state observations.

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