THE USE OF ENZYMES IN STRUCTURAL STUDIES ON POLYSACCHARIDES: I. THE MODE OF ATTACK OF A β-D-(l → 3)-GLUCANASE ON LAMINARIN
- 1 July 1963
- journal article
- research article
- Published by Canadian Science Publishing in Canadian Journal of Chemistry
- Vol. 41 (7) , 1671-1678
- https://doi.org/10.1139/v63-241
Abstract
A β-D-(1 → 3)-glucanase from the Basidiomycete sp. QM 806 of Reese and Mandels has been purified and shown to hydrolyze insoluble laminarin by removing glucose residues one at a time from the non-reducing end of the polysaccharide. The enzyme will also hydrolyze simple oligosaccharides and its mode of attack on these is similar to its action on laminarin. The products produced by the action of this preparation on laminarin are consistent with recent information on the structure of this polysaccharide.Keywords
This publication has 8 references indexed in Scilit:
- Studies on the metabolism of the Chrysophyceae. Comparative structural investigations on leucosin (chrysolaminarin) separated from diatoms and laminarin from the brown algaeBiochemical Journal, 1961
- SELECTIVE ENZYMOLYSIS OF POLY-β-D-GLUCANS, AND THE STRUCTURE OF THE POLYMERSCanadian Journal of Chemistry, 1960
- β-D-1, 3 GLUCANASES IN FUNGICanadian Journal of Microbiology, 1959
- The action of culture filtrates of the fungus Myrothecium verrucaria on β-glucosansBiochemical Journal, 1956
- Colorimetric Method for Determination of Sugars and Related SubstancesAnalytical Chemistry, 1956
- NOTES ON SUGAR DETERMINATIONJournal of Biological Chemistry, 1952
- The chromatographic determination of raffinose in raw sugars. Improvements in techniqueThe Analyst, 1952
- Detection of Sugars on Paper ChromatogramsNature, 1950