Triple staining for simultaneous visualization of cell types in islet of Langerhans of pancreas. Successive application of argyrophil, aldehyde-fuchsin and lead-hematoxylin stains in a single tissue section.

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Abstract
For differential staining of 3 types of endocrine cells (A, B and D) in a pancreatic islet, Grimelius'' silver nitrate stain is used for A cell, Gomori''s aldehyde-fuchsin stain for B cell and lead-hematoxylin stain for D cell. The stains give different colors to each cell type, and the techniques applied are simple and reliable. The staining sequences for preparing a triply stained [human] pancreatic section are described.

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