HIGH RESOLUTION SHADING CORRECTION
- 1 July 1974
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 22 (7) , 751-754
- https://doi.org/10.1177/22.7.751
Abstract
Quantitative cytology requires accurate representation of a specimen's optical densities. As the requirements for measurement precision increase, instrument-induced errors become increasingly more difficult to reduce to the point at which their effect on experimental data is insignificant compared to the measured parameters. Shading induces a significant amount of amplitude ambiguity to data obtained from a scanning system. A method of shading correction on single pixels is introduced as a new way to reduce some errors that currently plague scanning systems.Keywords
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