Bacteriorhodopsin Conjugates as Anchors for Supported Membranes
- 1 July 2004
- journal article
- research article
- Published by American Chemical Society (ACS) in Bioconjugate Chemistry
- Vol. 15 (4) , 942-947
- https://doi.org/10.1021/bc034231h
Abstract
The sophistication of supported lipid bilayer membranes has increased steadily as new applications are being explored. In general, tethered lipids are used to anchor the lipid bilayer to the substrate. Here we describe a new type of anchoring system for supported lipid bilayers that is based on biotin−PEG3400−bacteriorhodopsin conjugates. Amine-based coupling was used to construct the polymer conjugates, followed by fluorophore labeling to enable confocal imaging. The bacteriorhodopsin-based anchoring system was used to construct solid-supported vesicles from streptavidin-coated microspheres. This method could provide a new route for the stability enhancement of supported lipid bilayer membrane assemblies.Keywords
This publication has 5 references indexed in Scilit:
- Measuring Distances in Supported Bilayers by Fluorescence Interference-Contrast Microscopy: Polymer Supports and SNARE ProteinsBiophysical Journal, 2003
- Reconstituted Syntaxin1A/SNAP25 Interacts with Negatively Charged Lipids as Measured by Lateral Diffusion in Planar Supported BilayersBiophysical Journal, 2001
- Tethered Polymer-Supported Planar Lipid Bilayers for Reconstitution of Integral Membrane Proteins: Silane-Polyethyleneglycol-Lipid as a Cushion and Covalent LinkerBiophysical Journal, 2000
- Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 1. Solubilization of large unilamellar liposomes (prepared by reverse-phase evaporation) by Triton X-100, octyl glucoside, and sodium cholateBiochemistry, 1988
- Specific labelling of the protein and lipid on the extracellular surface of purple membraneJournal of Molecular Biology, 1978