Nickel Inhibits Binding of α2-Macroglobulin-Methylamine to the Low-Density Lipoprotein Receptor-Related Protein/α2-Macroglobulin Receptor but Not the α2-Macroglobulin Signaling Receptor
- 1 October 1997
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 36 (41) , 12395-12399
- https://doi.org/10.1021/bi970806k
Abstract
A previous study demonstrated that activated α2-macroglobulin (α2M*) binding to the low-density receptor-related protein/α2-macroglobulin receptor (LRP/α2MR) is blocked by Ni2+ [Hussain, M. M., et al. (1995) Biochemistry34, 16074−16081]. We now report that the effect of Ni2+ is on a region of the α2M molecule upstream of the carboxyl terminal receptor recognition domain. This observation is consistent with previous observations from this laboratory suggesting that α2M* binding to LRP/α2MR involves a region of the α2M molecule immediately upstream of the receptor recognition domain [Enghild, J. J., et al. (1989) Biochemistry28, 1406−1412]. We further demonstrate that Ni2+ has no effect on the binding of α2M* or a cloned and expressed receptor binding fragment (RBF) to the recently described α2M signaling receptor as assessed by direct binding and signal transduction studies.Keywords
This publication has 18 references indexed in Scilit:
- Up-regulation of the α2-Macroglobulin Signaling Receptor on Rheumatoid Synovial FibroblastsJournal of Biological Chemistry, 1997
- Identification of residues in α-macroglobulins involved in activation of the α2-macroglobulin signaling receptorBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1996
- Characterization of α2-Macroglobulin Binding to Human Trabecular Meshwork Cells: Presence of the α2-Macroglobulin Signaling ReceptorArchives of Biochemistry and Biophysics, 1996
- Selective Mutations in Cloned and Expressed α-Macroglobulin Receptor Binding Fragment Alter Binding to Either the α2-Macroglobulin Signaling Receptor or the Low Density Lipoprotein Receptor-related Protein/α2-Macroglobulin ReceptorJournal of Biological Chemistry, 1996
- Altered interaction of Cis-dichlorodiammineplatinum(II)--modified alpha 2-macroglobulin (alpha 2M) with the low density lipoprotein receptor-related protein/alpha 2M receptor but not the alpha 2M signaling receptor.Journal of Clinical Investigation, 1996
- STRUCTURES AND FUNCTIONS OF MULTILIGAND LIPOPROTEIN RECEPTORS: Macrophage Scavenger Receptors and LDL Receptor-Related Protein (LRP)Annual Review of Biochemistry, 1994
- Interaction of urokinase‐type plasminogenactivator (u‐PA) with its cellular receptor (u‐PAR) induces phosphorylation on tyrosine of a 38 kDa proteinFEBS Letters, 1993
- Expression of a functional α‐macroglobulin receptor binding domain in Escherichia coliFEBS Letters, 1992
- Evidence that the newly cloned low‐density‐lipoprotein receptor related protein (LRP) is the α2‐macroglobulin receptorFEBS Letters, 1990
- Cadmium binding to human α2-macroglobulinBiochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984