Pitfalls in the in vitro determination of sunscreen protection factors using broad band ultraviolet radiation detectors and solar simulating radiation

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Abstract
Reliable in vitro techniques for assessing sunscreen protection factors have relied upon obtaining the forward scattering transmission spectrum of the test compound applied to a suitable substrate such as mouse or human epidermis. A theoretical analysis of alternative techniques using broad-band ultraviolet radiation detectors in conjunction with a source of simulated sunlight shows that the method is likely to yield unreliable values of sun protection factors and the reasons for this are discussed.

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