FACTORS AFFECTING THE INVITRO ASSESSMENT OF OPSONIZATION - A STUDY OF THE KINETICS OF OPSONIZATION USING THE TECHNIQUE OF PHAGOCYTIC CHEMI-LUMINESCENCE

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 41  (4) , 903-911
Abstract
The importance of several factors involved in the investigation of opsonic defects was studied using phagocytic luminol-dependent chemiluminescence. The range for zymosan and bakers yeast [Saccharomyces cerevisae] opsonization by serum from healthy individuals was wide and kinetic studies showed comparative differences for different incubation periods, serum concentrations and particles. Decay in the serum opsonic activity stored at different temperatures was demonstrated and its clinical implications emphasized. By using techniques to ablate independently the classical and alternative complement activation pathways, the contribution of these to the opsonization of zymosan, Staphylococcus aureus (NCTC 6571), Pseudomonas aeruginosa and group B streptococcus (NCTC 11080) by normal and hypogammaglobulinemic serum at a concentration of 7% was assessed. By comparison of the results obtained for different incubation periods between particle and serum, the need for consideration of this parameter when assessing opsonic activity was shown. The results using the chemiluminescence assay were compared with those using other methods and correlated well.