Primary organization of nucleosome core particle of chromatin: sequence of histone arrangement along DNA.

Publisher Website
Google Scholar
Abstract
A high-resolution map for the arrangement of histones along DNA in the nucleosome core particle was determined by a sequencing procedure based on crosslinking histones to the 5''-terminal DNA fragments produced by scission of one DNA strand at the point of crosslinking. H1-depleted chromatin of Ehrlich ascity tumor cells were used to obtain the nucleosome core particles. The position of histones on DNA was identified by measuring the length of crosslinked DNA fragments. Each of the histones is arranged within several adjacent or dispersed DNA segments of a little less than 10 nucleotides in length. Histone-free intervals are located between these segments at the regular distances of about (10)n nucleotides from the 5'' end of the DNA and are likely to face 1 side of the DNA helix. Histones appear to be arranged in a similar manner on both DNA strands and do not form locks around DNA. A linearized model of the core particle is proposed.