Catalytic Role of the α-Carboxylate of the Glu Residue of Glutathione in Glutathione S-transferases
- 1 October 1996
- journal article
- research article
- Published by Taylor & Francis in Journal of Biomolecular Structure and Dynamics
- Vol. 14 (2) , 231-233
- https://doi.org/10.1080/07391102.1996.10508112
Abstract
The recently proposed catalytic role of the α-carboxylate of the Glu residue of glutathione in glutathione S-transferases (Widersten et al, Biochemistry 35, 7731–7742 (1996)) was examined. Based on structural considerations, it is clear that conformational changes in both glutathione and glutathione S-transferase are required. Recent kinetic studies by Ricci and coworkers (Ricci et al, J. Biol. Chem. 271, 16187–16192 (1996) and Caccuri et al, J. Biol. Chem. 271, 16193–16198 (1996)) may provide the missing evidence for these conformational changes. Possible ways to test this hypothesis are discussed.Keywords
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