A SOLID-PHASE RADIOIMMUNOASSAY FOR OVINE FOLLICLE-STIMULATING HORMONE

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Abstract
SUMMARY: A radioimmunoassay using antibody-coated polystyrene tubes is described, which is suitable for the estimation of follicle-stimulating hormone (FSH) levels in sheep blood plasma. Ovine FSH was labelled with 125I by an enzymic method employing lactoperoxidase and H2O2, and was stable for several months at −15 °C. Antisera to ovine FSH were raised in rabbits. Standard curves for unlabelled FSH obtained by this method with different preparations of labelled FSH showed reproducible slopes from day to day. Ovine luteinizing hormone (LH) and thyroid-stimulating hormone (TSH) showed about 17% cross-reaction in the assay, necessitating the application of a correction to the FSH values for samples of plasma containing more than 15 ng of LH or TSH/ml. The FSH content of plasma from wethers and anoestrous ewes ranged from 20 to 120 ng/ml.

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