PURIFICATION OF HLA ANTIGENS FROM URINE
- 1 November 1976
- journal article
- research article
- Published by Wolters Kluwer Health in Transplantation
- Vol. 22 (5) , 500-507
- https://doi.org/10.1097/00007890-197611000-00014
Abstract
HLA antigens were purified from urines of kidney transplanted patients. HLA was recovered as a single peak of 45,000 mol wt that was dissociated into β2-microglobulin and a 33,000 mol wt fraction bearing the allospecificity. The purified fractions contain carbohydrates but no lipids. Electrophoretical mobility and the relative salt concentration of eluting buffers in DEAE-Sephadex chromatography were determined for six antigens of the A locus and seven antigens of the B locus. Isolectric points of antigens A1, A2, A9, and B12 were measured. Physicochemical characteristics of HLA purified from urine appear to be similar to those of papain-solubilized cell membrane HLA. Urinary HLA was shown to originate from serum and not from renal or ureteric tissue.This publication has 0 references indexed in Scilit: