Autoradiographic Studies of the Differential Incorporation of Glycine, and Purine and Pyrimidine Ribosides by Paramecium aurelia

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Abstract
Autoradiographs were prepared from organisms of Paramecium aurelia which were grown in an axenic medium to which one of the following compounds was added: [carboxy-14C]glycine, [8-14c]adenosine, [8-14c]-guanosine, [2-14iC]cytidine, [3H]thymidine, and [3H]uridine. Trichloroacetic acid extracts of organisms grown with the isotopically labelled glycine, adenosine and cytidine were prepared and the radioactivity found either in the protein or in the acid-soluble fractions was determined in a scintillation counter. P. aurelia was unable to incorporate glycine into the nucleic acids of the macro- nucleus; however, this compound was utilized in the formation of the cytoplasmic proteins. Thymidine was preferentially taken up by the macronucleus; uridine was found mainly in the cytoplasm. The other purine and pyrimidine ribosides were found both in the macronucleus and cytoplasm.

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