ROLE OF RNA-METABOLISM IN POLYMORPHONUCLEAR LEUKOCYTE PHAGOCYTOSIS

  • 1 January 1977
    • journal article
    • research article
    • Vol. 90  (1) , 118-124
Abstract
The role of RNA metabolism in human PMN [polymorphonuclear] phagocytosis was examined. By the inhibition of RNA synthesis with actinomycin D, intracellular killing of a strain of actinomycin D-resistant Staphylococcus epidermidis was unaffected by 1 h but significantly decreased by 2 and 3 h. After 1 h, phagocytosis began to decrease, and iodination of proteins also decreased in the presence of actinomycin D. Hexosemonophosphate shunt activity remained similar for both actinomycin D treated PMN and control cells at 30 min and 2 h. Protein synthesis was inhibited by incubating PMN with puromycin. The ability of PMN to kill puromycin-resistant S. epidermidis was significantly decreased at 2 and 3 h.

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