Fluorometric, Chromatographic, and Spectronic Evidence for the Non-indolic Nature ofCitrusAuxin

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Abstract
Evidence for the non-indolic nature of the new citrus auxin is presented on the basis of fluorometric properties, thin-layer chromatography, Ehrlich's colour reaction, paper electrophoresis, and the infra-red spectra determinations. Citrus auxin had a lower Rf in TLC than IAA, did not give the typical indole reaction with Ehrlich's reagent, and behaved differently in electrophoresis. The infra-red spectra also provided preliminary information concerning chemical structure. The hypothesis that indolic compounds constitute the only natural auxins in higher plants should be revised in view of this evidence that a non-indole auxin exists in higher plants.

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