Gateway-Based Destination Vectors for Functional Analyses of Bacterial ORFeomes: Application to the Min System inBrucella abortus
- 15 February 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (4) , 1375-1379
- https://doi.org/10.1128/aem.01873-06
Abstract
Twenty Gateway-compatible destination vectors were constructed. The vectors comprise fluorescent and epitope fusion tags, various drug markers, and replication origins that should make them useful for exploring existing microbial ORFeomes. In an attempt to validate several of these vectors, we observed polar and oscillating localization of MinD in Brucella abortus.Keywords
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