New Way of Regulating Alternative Splicing in Retroviruses: the Promoter Makes a Difference
- 1 April 2007
- journal article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 81 (7) , 3652-3656
- https://doi.org/10.1128/jvi.02105-06
Abstract
Alternative splicing has been recognized as a major mechanism for creating proteomic diversity from a limited number of genes. However, not all determinants regulating this process have been characterized. Using subviral human immunodeficiency virus (HIV) env constructs we observed an enhanced splicing of the RNA when expression was under control of the cytomegalovirus (CMV) promoter instead of the HIV long terminal repeat (LTR). We extended these observations to LTR- or CMV-driven murine leukemia proviruses, suggesting that retroviral LTRs are adapted to inefficient alternative splicing at most sites in order to maintain balanced gene expression.Keywords
This publication has 33 references indexed in Scilit:
- Overcoming promoter competition in packaging cells improves production of self-inactivating retroviral vectorsGene Therapy, 2006
- Acetylated Tat Regulates Human Immunodeficiency Virus Type 1 Splicing through Its Interaction with the Splicing Regulator p32Journal of Virology, 2006
- An Exonic Splicing Silencer Downstream of the 3′ Splice Site A2 Is Required for Efficient Human Immunodeficiency Virus Type 1 ReplicationJournal of Virology, 2005
- Self-inactivating retroviral vectors with improved RNA processingGene Therapy, 2004
- MAZ Elements Alter Transcription Elongation and Silencing of the Fibroblast Growth Factor Receptor 2 Exon IIIbJournal of Biological Chemistry, 2004
- Human Immunodeficiency Virus Type 1 hnRNP A/B-Dependent Exonic Splicing Silencer ESSV Antagonizes Binding of U2AF65 to Viral Polypyrimidine TractsMolecular and Cellular Biology, 2003
- A Slow RNA Polymerase II Affects Alternative Splicing In VivoMolecular Cell, 2003
- Human p32 protein relieves a post-transcriptional block to HIV replication in murine cellsNature Cell Biology, 2003
- The proline-rich region of the ecotropic Moloney murine leukaemia virus envelope protein tolerates the insertion of the green fluorescent protein and allows the generation of replication-competent virusJournal of General Virology, 2003
- A genomic view of alternative splicingNature Genetics, 2002