Clinical Evaluation of a Type III Secretion System Real-Time PCR Assay for Diagnosing Melioidosis
- 1 August 2006
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 44 (8) , 3028-3030
- https://doi.org/10.1128/jcm.00913-06
Abstract
A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.Keywords
This publication has 12 references indexed in Scilit:
- INDIRECT HEMAGGLUTINATION ASSAY IN PATIENTS WITH MELIOIDOSIS IN NORTHERN AUSTRALIAThe American Journal of Tropical Medicine and Hygiene, 2006
- Development and Evaluation of a Real-Time PCR Assay Targeting the Type III Secretion System of Burkholderia pseudomalleiJournal of Clinical Microbiology, 2006
- Short report: application of a polymerase chain reaction to detect Burkholderia pseudomallei in clinical specimens from patients with suspected melioidosis.2005
- APPLICATION OF A POLYMERASE CHAIN REACTION TO DETECT BURKHOLDERIA PSEUDOMALLEI IN CLINICAL SPECIMENS FROM PATIENTS WITH SUSPECTED MELIOIDOSISThe American Journal of Tropical Medicine and Hygiene, 2005
- Rapid Immunofluorescence Microscopy for Diagnosis of MelioidosisClinical and Vaccine Immunology, 2005
- Melioidosis: Epidemiology, Pathophysiology, and ManagementClinical Microbiology Reviews, 2005
- MelioidosisThe Lancet, 2003
- Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCRJournal of Clinical Microbiology, 2001
- Comparison of three PCR primer sets for diagnosis of septicemic melioidosisActa Tropica, 2000
- Prognostic Significance of Quantitative Bacteremia in Septicemic MelioidosisClinical Infectious Diseases, 1995