Pyrosequencing as a Method for Grouping of Listeria monocytogenes Strains on the Basis of Single-Nucleotide Polymorphisms in the inlB Gene
Open Access
- 1 November 2001
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 67 (11) , 5339-5342
- https://doi.org/10.1128/aem.67.11.5339-5342.2001
Abstract
By using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of Listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlB gene. Strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups.Keywords
This publication has 17 references indexed in Scilit:
- Molecular grouping of Listeria monocytogenes based on the sequence of the inlB geneJournal of Medical Microbiology, 2000
- Surface protein p104 is involved in adhesion of Listeria monocytogenes to human intestinal cell line, Caco-2Journal of Medical Microbiology, 1999
- A Sequencing Method Based on Real-Time PyrophosphateScience, 1998
- Molecular characterization of an autolytic amidase of Listeria monocytogenes EGDMicrobiology, 1998
- WHO study on subtyping Listeria monocytogenes: results of phage-typingInternational Journal of Food Microbiology, 1996
- Entry of Listeria monocytogenes into hepatocytes requires expression of InIB, a surface protein of the internalin multigene familyMolecular Microbiology, 1995
- Restriction fragment length polymorphism in four virulence-associated genes of Listeria monocytogenesResearch in Microbiology, 1992
- Entry of L. monocytogenes into cells is mediated by internalin, a repeat protein reminiscent of surface antigens from gram-positive cocciCell, 1991
- ListeriosisJAMA, 1989
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977