Chlorophyll fluorescence quenching in isolated light harvesting complexes induced by zeaxanthin
Open Access
- 5 April 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 471 (1) , 71-74
- https://doi.org/10.1016/s0014-5793(00)01369-7
Abstract
Non‐photochemical quenching of chlorophyll fluorescence in plants occurs in the light harvesting antenna of photosystem II and is regulated by the xanthophyll cycle. A new in vitro model for this process has been developed. Purified light harvesting complexes above the detergent critical micelle concentration have a stable high fluorescence yield but a rapidly inducible fluorescence quenching occurs upon addition of zeaxanthin. Violaxanthin was without effect, lutein and antheraxanthin induced a marginal response, whereas the violaxanthin analogue, auroxanthin, induced strong quenching. Quenching was not caused by aggregation of the complexes but was accompanied by a spectral broadening and red shift, indicating a zeaxanthin‐dependent alteration in the chlorophyll environment.Keywords
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