Characterization of glutathione conjugates by fast atom bombardment/tandem mass spectrometry
- 1 June 1988
- journal article
- research article
- Published by Wiley in Journal of Mass Spectrometry
- Vol. 15 (11) , 615-621
- https://doi.org/10.1002/bms.1200151107
Abstract
The collisionally activated decomposition of [M + H]+ ions, generated by fast atom bombardment (FAB) of glutathione conjugates, has been studied by tandem mass spectrometry (MS/MS) using hybrid sector/quadrupole instruments. Abundant fragments of diagnostic utility were present in the daughter ion spectra. Common fragmentation modes were observed but their relative importance was strongly dependent on the nature of the conjugated species. As an example of a general approach to the characterization of glutathione conjugates in biological samples, the acetaminophen‐glutathione conjugate was identified in rat bile, following coadministration of (2H0)‐ and (2H3)acetaminophen, using the experimental sequence: (i) conventional FAB mass spectrometric analysis, (ii) MS/MS using constant neutral loss (129 u) scanning to identify parent ions corresponding to glutathione conjugates, (iii) MS/MS to yield daughter ion spectra of parents so identified and corresponding to (2H0)‐ and (2H3)‐labeled conjugates.This publication has 21 references indexed in Scilit:
- Application of fast atom bombardment mass spectrometry to biological samples: Analysis of urinary metabolites of acetaminophenJournal of Mass Spectrometry, 1984
- Morphine metabolism revisited. II. Isolation and chemical characterization of a glutathionylmorphine adduct from rat liver microsomal preparationsChemico-Biological Interactions, 1984
- Field desorption mass spectrometric characterization of thiol conjugates related to the oxidative metabolism of the anticancer drug 4′-(9-acridinylamino)-methanesulfon-m-anisidideJournal of Mass Spectrometry, 1983
- Some mass-spectral and n.m.r. analytical studies of a glutathione conjugate of aflatoxin B1Biochemical Journal, 1983
- Formation and identification of glutathione conjugates from 2-nitrosofluorene and N-hydroxy-2-aminofluoreneChemico-Biological Interactions, 1982
- Identification of glutathione conjugates formed from N-hydroxy-2-acetylaminofluorene in the ratChemico-Biological Interactions, 1982
- Identification of conjugation and cleavage products in the thiolytic metabolism of the anticancer drug 4′-(9-acridinylamino)methanesulfon-m-anisidideJournal of Mass Spectrometry, 1981
- Comparative electron impact, chemical ionization and field desorption mass spectra of some thioether metabolites of acetaminophenJournal of Mass Spectrometry, 1981
- Multi-step metabolic activation of benzene. Effect of superoxide dismutase on covalent binding to microsomal macromolecules, and identification of glutathione conjugates using high pressure liquid chromatography and field desorption mass spectrometryChemico-Biological Interactions, 1980
- Pentachloronitrobenzene metabolism in peanut. 1. Mass spectral characterization of seven glutathione-related conjugates produced in vivo or in vitroJournal of Agricultural and Food Chemistry, 1980