Measurement of serum unconjugated estriol and estradiol by high-performance liquid chromatography

Publisher Website
Google Scholar
Abstract
Measurement of Estriol (E3) and estradiol (E2) within 22 min by high‐performance liquid chromatography (HPLC) was achieved in this study, and the values were compared with those of a radioimmunoassay (RIA). A totally computerized HPLC method was developed for measuring unconjugated E3(u‐E3) and E2 (u‐E2) in the sera of pregnant women. The serum samples were injected directly into the apparatus and transferred to a pre‐treatment column where the estrogens were absorbed while hydrophilic components such as proteins and carbohydrates were excluded. The estrogens were then passed through another separation column containing a new type of polymer gel. The mobile phase consisted of an acetonitrile‐water mixture, and separation was achieved by means of a reversed‐phase procedure. The eluate was monitored for fluorescence. All procedures were monitored and controlled with a built‐in microcomputer. Serum samples from 97 normal pregnant women at 20–41 wk gestation were simultaneously assayed by HPLC and RIA. The correlations obtained by HPLC and RIA were as follows: u‐E3 y = 0.905x–0.385, with a coefficient of correlation of r = 0.912; for u‐E2, y = 0.964x + 5.024, with a coefficient of correlation of r = 0.841 (y, RIA value; x, HPLC value). The quick measurement of u‐E3 and u‐E2 by HPLC can be a useful method for evaluating fetoplacental function.

This publication has 12 references indexed in Scilit: