Monoclonol antibody against murine T cell receptor Vα14 cross-reacts with human CD3θ and detects disulfide-linked dimeric form
- 1 October 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in International Immunology
- Vol. 3 (10) , 991-995
- https://doi.org/10.1093/intimm/3.10.991
Abstract
A monoclonal antibody against V14+ α-chain of murine T cell receptor (TCR) was established by fusing spleen cells from a rat immunized with a soluble chimeric TCR/lgG3 protein containing murine TCR Vα14Jα281 in place of the VHDHJH of an lgG3-λ1, and subjected to screening on a human transfectant (Jurkat variant) expressing the murine V14J281 α-chain. The anti-mouse Vα14 antibody precipitated TCRαβ molecules from Triton X-100-solubilized extracts of 125l-labeled murine thymocytes and spleen cells. Unexpectedly, the antibody showed cross-reactivity to the human CD3θe molecule and detected a disulfide-linked 20 kDa dimeric form of human CD3ε, which is a novel family component of the CD3 complex and is associated closely with the CD3ζ-ζ homodimer as well as TCRαβ or TCRγθ.Keywords
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