Preparation and Properties of Immobilized Naringinase Using Tannin-aminohexyl Cellulose

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Abstract
Naringinase from Aspergillus niger was immobilized with 58 to 100% yield by adsorbing the enzyme to tannin-aminohexyl cellulose prepared by reaction of aminohexyl cellulose and cyanogen bromide activated Chinese gallotannin. Some enzymatic properties of the immobilized naringinase were investigated and compared with those of the native enzyme. The optimal pH (4~5) and optimal temperature (50°C) for enzyme reaction by the immobilized enzyme were the same as those of the native enzyme. The heat stability of the immobilized enzyme was better than that of the native naringinase. No significant difference of the Michaelis constant was detected. The operational stability of the immobilized naringinase column was investigated at 25 °C and 37°C by passing a naringin solution and Natsudaidai juice continuously through it. In the case of a naringin solution, the activity of the immobilized enzyme column was very stable and its half-life was estimated to be 198 days at 25°C and 88 days at 37°C. However, in the case of Natsudaidai juice, the half-life of immobilized column was estimated to be 45 days at 25°C and 15 days at 37°C.

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