ESTERASE OF THE SALIVARY GLANDS

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Abstract
Histochemical study of the distribution of esterase activity in the salivary glands of mouse, rat, and man was made on sections of frozen-dried embedded tissues. Azo-coupling methods for esterase activity were made with the following substrates: naphthyl acetate, naphthol AS acetate, naphthyl butyrate, and naphthyl propionate. The reactions were char-acterized by interposition of certain potential esterase inhibitors which included DFP (diisopropyl fluorophosphate) (10-5 [image]), sodium fluoride (.002 [image]), eserine (10-4 [image]), and Phemerol (benzethonium chloride) (.01 [image]). Activity with all substrates was similar. Sites of highest activity were serous cells of the tongue (rodent), and duct epithelium (all species). The "mucoid" cells of the submaxillary gland (rodent) were active, while mucous cells of the tongue and sublingual gland were inactive. Demilune cells exhibited activity in the following: mouse and rat tongue (mucous cells), rat sublingual gland, and human sublingual gland. Sodium fluoride, DFP, and Phemerol acted as inhibitors while eserine was without effect.

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