Immunogenicity of Leptospiral Vaccines Grown in Protein-free Medium

Abstract
Leptospira interrogans serovars pomona and hardjo were adapted to grow in a chemically-defined, protein-free (PF) medium. Formolized monovalent vaccines of serovars pomona and hardjo and a bivalent mixture of the two were prepared from PF cultures. Live PF cultures and the vaccine preparations retained their agglutinating antigens and their immunogenicity when tested in rabbits and guinea-pigs. The vaccines were not pyrogenic; dermal reactions were slight.

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