Gene cloning in Aspergillus nidulans: isolation of the isocitrate lyase gene (acuD)
- 1 February 1986
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 202 (2) , 271-275
- https://doi.org/10.1007/bf00331649
Abstract
An Aspergillus nidulans gene library was constructed in a high-frequency transformation vector, pDJB3, based on the Neurospora crassa pyr4 gene. This gene library was used to isolate the structural gene for isocitrate lyase (acuD) by complementation of a deficiency mutation following transformation of A. nidulans. Plasmids rescued in Escherichia coli were able to transform five different A. nidulans acuD mutants. Transformation using plasmids containing the cloned fragment resulted in integration at the acuD locus in six of nine transformants.Keywords
This publication has 30 references indexed in Scilit:
- Studies on transformation of Escherichia coli with plasmidsPublished by Elsevier ,2006
- Development of a high-frequency transforming vector for Aspergillus nidulansGene, 1985
- Transformation by integration in Aspergillus nidulansGene, 1983
- Transformation of Aspergillus nidulans by the orotidine-5′-phosphate decarboxylase gene of Neurospora crassaBiochemical and Biophysical Research Communications, 1983
- A reliable method for the recovery of DNA fragments from agarose and acrylamide gelsAnalytical Biochemistry, 1981
- Trans-complementable copy-number mutants of plasmid ColE1Nature, 1980
- Analysis of Acetate Non-utilizing (acu) Mutants in Aspergillus nidulansJournal of General Microbiology, 1976
- Mutants of Aspergillus nidulans Impaired in Penicillin BiosynthesisJournal of General Microbiology, 1974
- Isocitrate lyase from Neurospora crassaBiochimica et Biophysica Acta (BBA) - Enzymology, 1974
- The two-way selection of mutants and revertants in respect of acetate utilization and resistance to fluoro-acetate in Aspergillus nidulansGenetics Research, 1965