Intramolekulare Wasserstoffverschiebung in der Hexosephosphatisomerase-Reaktion bei der photosynthetischen Stärkebildung in Chlorella
Open Access
- 1 June 1966
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung B
- Vol. 21 (6) , 557-562
- https://doi.org/10.1515/znb-1966-0612
Abstract
Chlorella pyrenoidosa was illuminated in the presence of glucose and fructose echa having carbon-14 and tritium markers. Starch and sucrose were degraded to obtain the intramulocular distribution of 14C and tritium. Glucose-1-14C-2-T was incorporated into the glucose of starch and into the glucose moiety of sucrose without change of the T/14C ratio. Fructose-1-14C-1-T (labelled stereospecifically in the isomerase position) was incorporated into starch, yielding predominately glucose-1-14C-2-T, whereas fructose-1-14C-1-T (labelled in the nonisomerase position) gave mainly glucose-1-14C-1-T. The results, in particular the transfer of tritium from C-1 of fructose to C-2 of glucose, are interpreted as being a strong indication for an intramolecular hydrogen transfer in the hexosephosphat-isomerase reaction in a multienzyme system of the photosynthetic starch biosynthesis in vivo, shielding the hexoses of T-exchange reactions with water.Keywords
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