Purification and Characterization of Soluble Human IL-6 Receptor Expressed in CHO Cells
- 1 October 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 108 (4) , 673-676
- https://doi.org/10.1093/oxfordjournals.jbchem.a123261
Abstract
An immunosorbent assay system to detect genetically engineered IL-6 receptor (IL-6R) was established, whereby soluble IL-6 receptor (sEL-6R) was detected in the culture medium when sIL-6R cDNA was transfected into COS1cells. A stably transformed Chinese hamster ovary (CHO) cell line constitutively expressing sIL-6R has been established. The recom-binant sIL-6R was purified to homogeneity by sequential filtration and chromatography of the culture medium. The recombinant aIL-6R augmented the sensitivity of Ml cells to IL-6 in growth inhibition assay in a dose-dependent manner. Furthermore, a radioisotope immunosorbent assay (RIA) utilizing recombinant sIL-6R was established which could detect IL-6 in a quantity as low as 10 ng/ml.Keywords
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