ELASTASE ACTIVITY OF ALVEOLAR MACROPHAGES - MEASUREMENTS USING SYNTHETIC SUBSTRATES AND ELASTIN
- 1 January 1976
- journal article
- research article
- Published by Elsevier
- Vol. 113 (1) , 25-30
- https://doi.org/10.1164/arrd.1976.113.1.25
Abstract
Hamster, rat, guinea pig and rabbit alveolar macrophage extracts were tested for elastase activity using elastin suspended in agar and 2 synthetic substrates, p-nitrophenyl N-tert-butyloxycarbonyl L-alaninate (NBA) and succinyl-L-alanyl-L-alanyl--alanine-p-nitroanilide (SLAPN). Activity against NBA was easily detectable, but there was no activity against SLAPN or against elastin-agar, although the assay procedures employing these substrates measured as little as 50 and 2 ng of pancreatic elastase, respectively. Unstimulated alveolar macrophages from these animals apparently do not contain elastase, and that NBA activity is misleading as an indicator of elastolytic activity in crude alveolar macrophage extracts from these species.This publication has 1 reference indexed in Scilit:
- Pulmonary Emphysema and Alpha1‐Antitrypsin DeficiencyActa Medica Scandinavica, 1964