Structure of Muramidase (Lysozyine)

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Abstract
Denaturation of muramidase in the increasing concentration of 2-chloroethanol, ethanol or dioxane was studied by determining viscosity, optical rotatory dispersion and difference spectrum. The results suggest the conformational changes as follows: (1) In the presence of increasing 2-chloroethanol concentration (16[image]32%), native muramidase is converted to a highly helical structure via an intermediate in which internal hydrophobic region is disrupted, as implied by a great spectral change; (2) In ethanol (60[image]80%) only the internal hydrophobic region is disrupted whereas the helical portions remain unchanged; (3) the internal hydrophobic region of muramidase molecule is lacking in any helical structure.