SV40 T-antigen is required for maintenance of immortal growth in SV40-transformed human fibroblasts.

1 January 1991

journal article
Published by Japan Society for Cell Biology in Cell Structure and Function

Vol. 16 (1) , 55-62
https://doi.org/10.1247/csf.16.55

Abstract

Two lines of immortal human fibroblasts were isolated following transfection of TIG-3 cells with plasmid DNA, pMT-1ODtsA, that contained SV40 early gene with a deletion in replication origin and ts mutation in coding sequence for T-antigen. These cells continued proliferation at 34 degrees C, over 565 population doubling level (PDL) which is far over the limited division potential of untransformed normal TIG-3 of 70-80 PDL. When the culture temperature was shifted to 40 degrees C after 70 PDL, they ceased proliferation immediately. One of these immortal clones, SVts8, lost its ts phenotype after retransformation with wtT-antigen gene. These results indicated that the function of intact T-antigen is required for maintenance of immortal proliferation, at least in one of the SV40 transformed immortal clones.

Keywords

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