Influence of the Structure of Chemically Bonded C18Phases on HPLC Separation of Naproxen Glucuronide Diastereoisomers

Abstract
On the basis of Nucleosil-100 a series of materials with varying structure and different coverage density of chemically bonded C18 phases (CBP) were prepared. The physico-chemical characteristics of these packings e.g. porosity and carbon content were studied by the BET method and CHN analysis. The structure of the C18 CBP was determined by solid state CP/MAS NMR. The prepared packings and columns have been applied for HPLC separation of naproxen and its two diastereoisomeric conjugates with glucuronic acid. Material with monomeric C18 CBP structure and with high coverage density has given good and reproducible separation results.