Monoclonal Antibodies toAfipia felis—A Putative Agent of Cat Scratch Disease
Open Access
- 1 May 1994
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 101 (5) , 603-606
- https://doi.org/10.1093/ajcp/101.5.603
Abstract
Twelve hybridoma clones producing monoclonal antibodies to Afipia felis, a putative causative agent of cat scratch disease, were derived from BALB/c mice immunized with A felis. All 12 monoclonal antibodies were species-specific for A felis and reacted with lipopolysaccharide antigens of A felis. These monoclonal antibodies belong to immunoglobulin (Ig) Gl, IgG2a, IgG2b, IgG3, and IgM isotypes. All monoclonal antibodies reacted with both agar-grown A felis and tissue culture-propagated A felis. Formalin fixation did not alter the reactivity of the antigen with the monoclonal antibodies. These monoclonal antibodies could be a useful tool for investigation of the disputed role of A felis in cat scratch disease.Keywords
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