Studies on Triglyceride Synthesis in Lipid Micelles from Adipose Tissue

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Abstract
Lipid micelles were prepared from isolated fat cells by treatment under hypotonic conditions as reported previously. The micelles were found to have triglyceride synthesizing activity as well as lipolytic activity. Adrenaline and dibutyryl cyclic AMP (DBcAMP) had no effect on triglyceride synthesis, but greatly stimulated lipolysis in the micelles. Calcium ions inhibited triglyceride synthesis and activated lipolysis. Triglyceride synthesizing activity was found in the pellet fraction obtained by centrifuging the micelles at 105,000 × g for 60 min. This fraction also contained lipase [EC 3.1.1.3] and esterase [EC 3.1.1.1] activities. Calcium ions inhibited the triglyceride synthesizing activity and activated the lipase activity of the pellet fraction. Based on these results, it was suggested that calcium ions may be important in the lipid metabolism of the micelles.

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