Determination of the intracellular sodium concentration in perfused mouse liver by31P and23Na magnetic resonance spectroscopy
- 1 January 1998
- journal article
- research article
- Published by Wiley in Magnetic Resonance in Medicine
- Vol. 39 (1) , 155-159
- https://doi.org/10.1002/mrm.1910390122
Abstract
A combination of 31P and 23Na NMR spectroscopy has been used to quantify the concentration of intracellular sodium, [Na]IC in the isolated and perfused mouse liver. The 31P resonances of dimethyl methylphosphonate and LaDOTP5−, markers of total tissue space and extracellular space, respectively, were used to determine the intracellular liver volume. For a mean wet weight of 1.7 ± 0.3 g, the intracellular liver volume as measured by 31P NMR averaged 1.2 ± 0.2 ml. The amount of intracellular sodium was measured from the baseline‐resolved intracellular 23Na resonance during perfusion of the shift reagent, TmDOTP5−. These two measurements resulted in an NMR‐determined value for [Na]IC of 29.0 ± 5.2 mM. Separate measurement of total tissue Tm and Na by atomic absorption spectroscopy on the same samples provided an AAS‐determined value for [Na]Ic of 32.1 ± 7.4 mM. These results indicate that intracellular sodium in the isolated, perfused liver is 100% visible by 23Na NMR spectroscopy.Keywords
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