Abstract
A study of the changes in the limonin monolactone content of Washington Navel oranges, grown on sour orange rootstock, from blossom until maturity was carried out. At the same time the changes in the limonin monolactone content in the leaves from the spring‐flush, adjoining the fruits used in the investigation, were studied. From the start of development of the fruits there was an important biosynthetic activity of limonin monolactone, as indicated by its accumulation up to a maximum of 47 mg per fruit—around 130 days after anthesis. Throughout the whole development of the fruit the total limonin monolactone content in the endocarp was found to be greater than in the peel. The limonin monolactone content in the leaves decreased gradually from the start of the experiment until 130 days after anthesis.

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