A novel olefinic rearrangement. The enzymic conversion of cholesta-7,9-dien-3β-ol into cholesta-8,14-dien-3β-ol
- 1 September 1972
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 129 (2) , 225-229
- https://doi.org/10.1042/bj1290225
Abstract
1. [3α-3H]Cholesta-7,9-dien-3β-ol is converted in high yield into cholesterol by a 10000gav. supernatant fraction of rat liver homogenate. 2. Incubation of cholesta-7,9-dien-3β-ol with [4-3H]NADPH and rat liver microsomal fractions under anaerobic conditions resulted in3H being incorporated into the 14α-position of cholest-7-en-3β-ol. 3. Under anaerobic conditions in the absence of NADPH cholesta-7,9-dien-3β-ol was isomerized into cholesta-8,14-dien-3β-ol by rat liver microsomal fractions.Keywords
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