STUDIES ON THE FERMENTATIVE PRODUCTION OF L-PROLINE

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Abstract
L-Proline production by an isoleucine auxotrophic mutant No. 14-5 (ATCC No. 15940), derived from glutamic acid-producing strain of B. flavum 2247, was investigated. This strain produces a large amount of L-proline under vigorous aeration at 31[degree] in a pH range from 7.0 to 7.5. Among various substances supplemented to the medium, the following substances enhance L-proline production: divalent cations, such as Fe++, Mn++, Zn++ and Mg++ (at rather high concentration); organic acids related to the tricarboxylic acid (TCA) cycle, and tartaric acid; some organic nutrients such as meat extract, "Ajieki" polypeptone and yeast extract; amino acids such as L-histidine, L-tryptophan, L-tyrosine, L-phenylalanine, glycine, L-serine, L-cystine, L-cysteine and L-ornithine. This microorganism lacks the threonine dehydratase activity, and a possible relation of the deficiency in threonine dehydratase activity in glutamic acid-producing bacteria to the abundant production of L-proline was discussed.

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