Amperometric Determination of Isoniazid in a flowing Stream at the Glassy Carbon Electrode

Publisher Website
Google Scholar
Abstract
A flow-injection method for the determination of isoniazid based on electrochemical oxidation at the glassy carbon electrode is presented. The amperometric method is highly specific and may be used to determine isoniazid in the presence of most other drugs and/or preservatives commonly found in its pharamaceutical dosage forms or administered concurently in therapeutic situations. Using an electrode potential of +825 mV, a calibration curve is linear (r = 0.9999) in the 0.05-6 μg/ml concentration range with minimum detectability at 0.5 ng (S/N = 2). The method applied to the analysis of isoniazid in a pharmaceutical dosage form shows good accuracy and precision. Although automation was not used in this study, the method could readily be incorporated in automated systems because it employs the technique of continuous analysis in a flowing stream.