Predicting coding function from nucleotide sequence or survival of "fitness" of tRNA.
- 1 June 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (6) , 3539-3543
- https://doi.org/10.1073/pnas.77.6.3539
Abstract
The sequence of a nucleotide region of f1 bacteriophage was determined on a bonded ultrathin acrylamide gel with a discontinuous buffer system by using the dideoxy-DNA sequencing method. This sequence and 1 other were analyzed for maximal base pairing with tRNA. The results allow a prediction of the direction and phase of possible coding functions. The implication of sequence constraints on mRNA codon frequency, tRNA structure, the origin of protein synthesis and triplet reading are discussed in terms of neutral, Darwinian and genotypic selectionist perspectives of evolution. The model of Crick, Brenner, Klug and Pieczenik (1976) for the origin of the genetic code is used to interpret contemporary adaptive and functional nucleic acid sequences.This publication has 23 references indexed in Scilit:
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