Micronuclei Formation in Tissue Cultured Cells Treated with Colchicine.

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Abstract
Formation of micronuclei and mitotic figures was quantitated with respect to time and concentration of colchicine. Tissue culture preparations were examined after staining with acridine orange by fluorescence microscopy. Mitotic figures increased linearly until hour 12. A rapid drop to control values at hour 15 was correlated with the appearence of micronucleated cells. A short lag period was followed by an enhanced rate of formation of mitotic figures. Except for shifts induced by this lag period, micronucleated cells increased after hour 12 at a constant rate. The micronucleated cells were considered terminal forms resulting from colchicine treatment. Our observation indicates that micronuclei are produced primarily by chromatid breakage and not by fragmentation of nuclei during interphase.

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