The Effects of Adulterating Agents on FPIA Analysis of Urine for Drugs of Abuse*

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Abstract
A variety of chemical agents were evaluated to determine their effects on fluorescence polarization immunoassays for drugs of abuse. Sixteen different agents, at concentrations up to 10%, were tested against urine assays for cannabinoids, cocaine (metabolite), amphetamines, opiates, phencyclidine (PCP), and barbiturates. The potential to cause both false positive and false negative results was evaluated, and assays were performed one and seven days after sample adulteration to simulate different collection/testing formats. All six drug assays were susceptible to one or more adulterating agents, but the degree varied considerably between assays. The cannabinoid assay was most susceptible to adulterant-induced false negative results, and the barbiturate assay was most susceptible to false positive results. The remaining assays demonstrated relatively few, but characteristic effects, some of which were attributable to drug degradation and others to assay interference. Although the results of pH measurement on adulterated samples verified its utility in identifying some samples adulterated with interfering agents, other adulterants that cause substantial effects would not be identified by pH measurements alone.

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